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Cell culture media for bioreactors are commonly prepared as aqueous solutions that provide the essential nutrients and conditions required for optimal cell growth, product yield, and quality. Even subtle variations in media composition can significantly impact cell growth rates and yield. Consequently, the identification and analysis of cell culture media are critical for maintaining consistency and optimizing performance.

In recent years, the pharmaceutical industry has increasingly turned to spectroscopic techniques such as fluorescence for cell culture media analysis. These methods offer advantages including rapid testing, minimal sample handling, and relatively lower costs compared to traditional approaches like mass spectrometry and chromatography. Notably, Fluorescence Excitation-Emission Matrix molecular fingerprinting, combined with simultaneous Absorbance and Transmission measurements (A-TEEM), has emerged as a powerful tool for analyzing media composition.