Fluorescence of Molecular Beacons
Biological processes can be traced through study of gene-expression via a 鈥渕olecular beacon鈥� (single-stranded DNA, ssDNA), a hairpin-shaped oligonucleotide with a fluorophore (donor) and a quencher (acceptor). The hairpin鈥檚 stem has two ends of complementary DNA (cDNA) that pair up. When hybridized, the fluorophore and quencher are close, producing little or no fluorescence. Molecular beacons are used to study enzyme interactions, cDNA sequencing, and biosensing.1
1 X. Liu, et al.,Anal. Biochem. 2000, 283, 56鈥� 63; X. Fang, et al., Anal. Chem. 2000, 72(14), 3280鈥�3285.
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