蓝鲸体育直播

Absorption Accessory

Add UV-VIS Spectrophotometry to your Fluorometer

蓝鲸体育直播 fluorometers have been recognized for their high sensitivity and ability to adapt to various applications due to their modular design. The addition of a simple absorption accessory enhances the performance and versatility of the instrument even more.

In many fluorescence experiments, it is necessary to know the absorption properties of the sample. The ABS-ACC absorption accessory from 蓝鲸体育直播 fits directly into a cuvette sample holder and enables the user to measure the absorption spectrum, or check the optical density, of a liquid sample without reconfiguring the fluorometer.

Absorption measurements are complementary to fluorescence. They are necessary for fluorescence quantum yield determination, and more accurate results are usually obtained by using the same optical path for
both fluorescence and absorption measurements. The ability to check the optical density during a fluorescence experiment makes it easy to avoid inner filter effects. By comparing the absorption and excitation spectra, one can draw conclusions about the purity of the sample.

This absorption accessory is very easy to use and enhances a 蓝鲸体育直播 fluorometer by offering more versatility. It eliminates the need for a separate, and often expensive, UV-VIS spectrophotometer.

Segment: Scientific
翱诲诲臎濒别苍铆: Fluorescence Spectroscopy
V媒robn铆 spole膷nost: 蓝鲸体育直播 Scientific

Inexpensive and easy-to-use accessory to achieve quality absorbance on 蓝鲸体育直播 fluorometers

ABS-ACC, perfect to avoid Inner Filter Effect

  • The fluorescence intensity is proportional to the intensity of the light source (actually proportional to the absorbed light intensity).
  • The linear relationship between fluorescence intensity and concentration holds only for dilute solutions where absorbance is lower than ~ 0.1 OD.
  • At high concentrations, light absorption can make the measured fluorescence intensity lower than the true fluorescence emission: This is called the Inner Filter Effect (IFE).
  • As a consequence, IFE limits the linearity of the fluorescence signal at higher sample concentrations due to primary and secondary re-absorption of fluorescence.
  • The ABS-ACC accessory is the perfect tool to monitor the concentration of the samples to avoid the inner filter effect by easily determining if the absorbance is too high.
Sample holderCompatible with cuvette 10 x 10 mm path length cuvette
ReflectorWavelength range 0.20 to 2.00 渭m
Minimum absorbance0.005
Maximum absorbance3.00
Mode of operationSingle beam
Wavelength rangeAccording to the Fluorometer configuration

The ABS-ACC absorption accessory is compatible with the following 蓝鲸体育直播 fluorometers.

  • 贵濒耻辞谤辞濒辞驳-蚕惭鈩
  • 贵濒耻辞谤辞濒辞驳庐-3
  • 狈补苍辞濒辞驳庐
  • 贵濒耻辞谤辞惭补虫庐

 

The ABS-ACC absorption accessory requires one of the following types of sample holder trays.

  • Standard room-temperature cuvette holder
  • Single position water jacketed cuvette holder
  • Single position thermo-electric cuvette holder

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